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Analysis of Rat Total MMP-9 by ELISA (CAT#: STEM-MB-1528-LGZ)

Introduction

Matrix metalloproteinase 9(MMP-9), also known as 92kDaIV type collagenase, 92kDa gelatinase, or gelatinase B, is an enzyme belonging to the Zinc-metalloproteinase family. MMP-9 is involved in the degradation of extracellular matrix in normal physiological processes (such as embryonic development, reproduction, angiogenesis, bone development, wound callus migration, learning and memory) and pathological processes (such as arthritis, cerebral hemorrhage, and tumor metastasis). MMP-9 and elastase appear to be regulators of neutrophil migration through the basement membrane. MMP-9 plays an important role in blood vessel and neovascularization. It is significantly upregulated during human respiratory epithelial healing and has been found to be associated with many pathological processes such as cancer, immune and cardiovascular diseases. Elevated levels of MMP-9 were found in cases of rheumatoid arthritis and focal cerebral ischemia. Its level increases with the development of idiopathic atrial fibrillation.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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