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Analysis of Relaxin-2 (Human) by ELISA (CAT#: STEM-MB-1158-LGZ)

Introduction

Relaxin-2, also called H2 relaxin, is a peptide hormone that plays an important role in female reproduction. It circulates in the blood as a dimer of disulfide-linked alpha and beta chains. Relaxin is best known as a labor hormone that promotes the growth and softening of the cervix, the development of the mammary glands, and has a pronounced effect on the uterus. In particular, it promotes angiogenesis, inhibits MMP production and activity, and downregulates estrogen receptor alpha expression.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Reproductive Biology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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