Analysis of RHBG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0619-LGZ)

Introduction

Official Full Name: Rh family B glycoprotein<br />Also known as: SLC42A2<br />This gene encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini. Alternative splicing of this gene results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PRADX Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2114-LGZ)
Analysis of TRPM5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2176-LGZ)
Analysis of H3C15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1055-LGZ)
Analysis of NRAS Gene (Mutation) in Noonan Syndrome by RT-qPCR (CAT#: STEM-MT-0129-LGZ)
Analysis of California Encephalitis Virus by Real-Time PCR Method (CAT#: STEM-MB-2717-LGZ)
Analysis of ACBD6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0778-LGZ)
Analysis of TRIM8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2094-LGZ)
Analysis of BTD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1830-LGZ)