Analysis of RIC3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2107-LGZ)

Introduction

Official Full Name: RIC3 acetylcholine receptor chaperone
Also known as: RIC-3; AYST720; PRO1385
This gene encodes a member of the cholinesterase 3-like inhibitor resistance family that acts as a chaperone for specific serotonin type 3 receptors and nicotinic acetylcholine receptor subtypes. The encoded proteins affect the folding and assembly of these receptor subunits in the endoplasmic reticulum and expression on the cell surface. The protein contains an N-terminal transmembrane domain, a proline-rich spacer, and a cytoplasmic C-terminal coiled domain. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Enterovirus (EV) - C by Real-Time PCR Method (CAT#: STEM-MB-4020-LGZ)
Analysis of Prolactinomas by RT-qPCR (CAT#: STEM-MT-0003-LGZ)
Analysis of KCNQ1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2258-LGZ)
Analysis of SPON1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2429-LGZ)
Analysis of PDE4DIP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0557-LGZ)
Analysis of Aeromonas Punctata by Real-Time PCR Method (CAT#: STEM-MB-3292-LGZ)
Analysis of OR51A7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2310-LGZ)
Analysis of TTC27 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1796-LGZ)