Analysis of RNF115 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0614-LGZ)

Introduction

Official Full Name: ring finger protein 115
Also known as: BCA2; ZFP364; ZNF364; RABRING7
Enables ubiquitin-protein transferase activity. Involved in negative regulation of epidermal growth factor receptor signaling pathway; protein autoubiquitination; and ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Predicted to be located in cytosol.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PROC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1471-LGZ)
Analysis of S100A11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0634-LGZ)
Analysis of Nosema Apis by Real-Time PCR Method (CAT#: STEM-MB-3806-LGZ)
Analysis of Avian Leukosis Virus (ALV) by Real-Time PCR Method (CAT#: STEM-MB-2847-LGZ)
Analysis of TMEM237 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1693-LGZ)
Analysis of DEDD Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0907-LGZ)
Analysis of Ehrlichia Risticii by Real-Time PCR Method (CAT#: STEM-MB-3351-LGZ)
Analysis of BIRC6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1795-LGZ)