Analysis of SCN2A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1484-LGZ)

Introduction

Official Full Name: sodium voltage-gated channel alpha subunit 2
Also known as: EA9; HBA; NAC2; BFIC3; BFIS3; BFNIS; DEE11; HBSCI; EIEE11; HBSCII; Nav1.2; SCN2A1; SCN2A2; Na(v)1.2
Voltage-gated sodium channels are transmembrane glycoprotein complexes consisting of a large alpha subunit and four repeat domains, each consisting of six transmembrane segments, and one or more regulatory beta subunits . Voltage-gated sodium channels play a role in the generation and propagation of action potentials in neurons and muscles. This gene encodes a member of the sodium channel alpha subunit gene family. Allelic variants of this gene have been associated with epilepsy and autism spectrum disorders. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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