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Analysis of SEMA4A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0147-LGZ)

Introduction

Also known as: RP35; SEMB; SEMAB; CORD10<br />This gene encodes a member of the signaling protein family of soluble and transmembrane proteins. Signaling proteins are involved in many functions including axon guidance, morphogenesis, oncogenicity and immune regulation. The encoded protein is a monoubiquitous type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing a local signal to designate areas inaccessible to growing axons. It is a T-cell-mediated immune activator that inhibits vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases, including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Polyp of colon, Retinitis pigmentosa, Cone-rod dystrophy 10

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements