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Analysis of Serpin E1 (inactive) (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0546-LGZ)

Introduction

Serpin E1, also known as plasminogen activator inhibitor-1 (PAI-1), is a member of a superfamily of serine protease inhibitors, produced primarily by the endothelium (cells lining the endothelium of blood vessels), but also secreted by other types of tissue such as adipose tissue. Serpin E1 is a major inhibitor of urokinase and tissue plasminogen activators (uPA and tPA).

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular, Cardiac Biomarkers, Growth Factors

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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