Analysis of Serratia liquefaciens by Real-Time PCR Method (CAT#: STEM-MB-3175-LGZ)

Introduction

Serratia liquefaciens is an organism rarely encountered in clinical practice. It belongs to the genus Serratia and the family Enterobacteriaceae. It is widely distributed in nature, including river water, mineral, spring and table water, domestic sewage, fish, minced meat and pasteurized milk or cream.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.