Analysis of SLC22A18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2118-LGZ)

Introduction

Official Full Name: solute carrier family 22 member 18
Also known as: HET; ITM; BWR1A; IMPT1; TSSC5; ORCTL2; BWSCR1A; SLC22A1L; p45-BWR1A
This gene is one of several tumor suppressor subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast. This gene is imprinted, with preferential expression from the maternal allele. Mutations in this gene have been found in Wilms tumors and lung cancers. This protein may act as a transporter of organic cations and play a role in the transport of chloroquine and quinidine-related compounds in the kidney. Several alternatively spliced transcript variants encoding different isoforms have been described.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PIK3R3 Gene (Mutation) in Familial Cancer of Breast by RT-qPCR (CAT#: STEM-MT-0105-LGZ)
Analysis of SRGAP2B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0682-LGZ)
Analysis of RNF223 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1411-LGZ)
Analysis of EGFL7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1995-LGZ)
Analysis of MRGPRX3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2418-LGZ)
Analysis of Kumlinge Virus by Real-Time PCR Method (CAT#: STEM-MB-3536-LGZ)
Analysis of AP4B1-AS1 Gene (Mutation) in Hereditary Spastic Paraplegia 47 by RT-qPCR (CAT#: STEM-MT-0126-LGZ)
Analysis of Shigella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3505-LGZ)