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Analysis of SLC22A18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2118-LGZ)

Introduction

Official Full Name: solute carrier family 22 member 18<br />Also known as: HET; ITM; BWR1A; IMPT1; TSSC5; ORCTL2; BWSCR1A; SLC22A1L; p45-BWR1A<br />This gene is one of several tumor suppressor subtransferable fragments located in the imprinted gene domain of 11p15.5, an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast. This gene is imprinted, with preferential expression from the maternal allele. Mutations in this gene have been found in Wilms tumors and lung cancers. This protein may act as a transporter of organic cations and play a role in the transport of chloroquine and quinidine-related compounds in the kidney. Several alternatively spliced transcript variants encoding different isoforms have been described.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements