Analysis of SMG5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0325-LGZ)
Introduction
Official Full Name: SMG5 nonsense mediated mRNA decay factor Also known as: EST1B; SMG-5; LPTSRP1; LPTS-RP1 SMG5 is involved in nonsense-mediated mRNA decay.
Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.
Applications
Gene mutation analysis.
Procedure
1. Sample processing and preparation of PCR reaction system. 2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument. 3. Set the program for PCR amplification. 4. Data analysis.
Materials
Sample: depends on the customer's analysis requirements