Analysis of SP-D/SFTPD/SFTP4 by ELISA (CAT#: STEM-MB-1587-LGZ)

Introduction

Surfactant protein D (SP-D), with a molecular weight of 43kDa, is a member of the collagen lectin family with innate immune regulation. It is secreted constitutively by alveolar lining cells and epithelial cells associated with tubular structures, and is induced in cardiac smooth muscle and endothelial cells. Human SP-D exists in serum, plasma, bronchoalveolar lavage fluid and amniotic fluid. SP-D plays an important role in the body's defense and innate immunity. SP-D binds neutrophil defensins and regulates antiviral defense. Current research shows that SP-D is involved in the occurrence and development of various lung diseases and can be used as a biomarker for these diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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