Analysis of STAT4 (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-1189-LGZ)

Introduction

Signal transducer and activator of transcription 4 (STAT4) is a transcription factor belonging to the STAT protein family, consisting of STAT1, STAT2, STAT3, STAT5A, STAT5B, and STAT6. STAT protein is the key activator of gene transcription, which binds to DNA under the action of cytokine gradient. STAT proteins are common components of the Janus kinase (JAK) signaling pathway, activated by cytokines. STAT4 is required for the development of Th1 cells from naive CD4+ T cells and the production of IFN-γ in response to IL-12. Two known STAT4 transcripts, STAT4α and STAT4β, differ in the level of downstream interferon-γ (IFN-γ) production.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Tissue homogenates, cell lysates and other biological fluids

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