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Analysis of Klotho (Human) by ELISA (CAT#: STEM-MB-1016-LGZ)

Introduction

Klotho is a transmembrane protein that regulates the sensitivity of organisms to insulin. It has β-glucuronidase activity and can hydrolyze β-glucuronic acid, which is related to human aging. Circulating klotox in plasma decreases gradually with age. β-Klotsole will cooperate with FGF receptors, and can bind to FGF19, FGF20, FGF23 and other fibroblast growth factors (FGF). The mechanism of action of Kloxol is still unknown, but it is known that it can regulate the calcium balance of cells by increasing the expression of TRPV5 and reducing the expression of TRPC6. In addition, Kloxol also increases the expression of inward rectifier ion channel ROMK.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Metabolic, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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