Analysis of Streptococcus Salivarius by Real-Time PCR Method (CAT#: STEM-MB-3140-LGZ)

Introduction

S. salivarius is a spherical, Gram-positive, facultative anaerobic commensal bacterium that is both catalase and oxidase negative. As one of the first colonizers of the human oral cavity, upper respiratory tract, and gut after birth, S. salivarius is thought to contribute to immune homeostasis and regulate inflammatory response. Thus, the bacterium is most often harmless but considered an opportunistic pathogen.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.