Analysis of Streptococcus Thermophilus by Real-Time PCR Method (CAT#: STEM-MB-3126-LGZ)

Introduction

Streptococcus thermophilus is a Gram-positive spherical to ovoid nonmotile coccus, 0.7–0.9 μm in diameter, occurring in pairs and chains, some of which can be very long. The bacterium has an optimum growth temperature of 40–45 °C, a minimum of 20–25 °C, and a maximum near 47–50 °C. <br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.