Analysis of SUMF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1820-LGZ)

Introduction

Official Full Name: sulfatase modifying factor 1<br />Also known as: FGE; UNQ3037; AAPA3037<br />This gene encodes an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TAFA3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1142-LGZ)
Analysis of D2HGDH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1570-LGZ)
Analysis of ABCB6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1515-LGZ)
Analysis of TNNI2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2146-LGZ)
Analysis of SFTPB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1685-LGZ)
Analysis of Ureaplasma Spp. by Real-Time PCR Method (CAT#: STEM-MB-3748-LGZ)
Overlap extension PCR (OE-PCR) (CAT#: STEM-MB-0215-WXH)
Analysis of Trichomonas Vaginali by Real-Time PCR Method (CAT#: STEM-MB-3184-LGZ)