Analysis of SYT6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1155-LGZ)

Introduction

Official Full Name: synaptotagmin 6<br />Also known as: sytVI<br />The protein encoded by this gene belongs to the synaptotagmin family. Synaptotagmins share a common domain structure that includes a transmembrane domain and a cytoplasmic region composed of 2 C2 domains, and are involved in calcium-dependent exocytosis of synaptic vesicles. This protein has been shown to be a key component of the secretory machinery involved in acrosomal exocytosis. Alternatively spliced transcript variants have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Human Rhinovirus 16 by Real-Time PCR Method (CAT#: STEM-MB-3272-LGZ)
Analysis of PSEN2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0612-LGZ)
Analysis of Schistosoma Bovis by Real-Time PCR Method (CAT#: STEM-MB-3368-LGZ)
Analysis of Brucella Neotomae by Real-Time PCR Method (CAT#: STEM-MB-2733-LGZ)
Analysis of Merkel Cell Polyomavirus (MCPyV) by Real-Time PCR Method (CAT#: STEM-MB-3823-LGZ)
Analysis of DVEV Ag by Real-Time PCR Method (CAT#: STEM-MB-2895-LGZ)
Analysis of Human Papillomavirus 16 (HPV-16) by Real-Time PCR Method (CAT#: STEM-MB-3347-LGZ)
Analysis of IARS2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1037-LGZ)