Analysis of TALDO1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2153-LGZ)

Introduction

Official Full Name: transaldolase 1<br />Also known as: TAL; TALH; TAL-H; TALDOR<br />Transaldolase 1 is a key enzyme in the pentose phosphate nonoxidative pathway, providing ribose 5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway also maintains the reduced state of glutathione, which protects thiols and cellular integrity from oxygen free radicals. The functional gene of transaldolase 1 is located on chromosome 11, and the pseudogene is located on chromosome 1, but there are conflicts in the map positions. The second and third exons of the gene develop through the insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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