Analysis of TERT Expression by RT-qPCR (CAT#: STEM-MT-0004-LGZ)

Introduction

Several somatic mutational hotspots have recently been identified in the telomerase reverse transcriptase (TERT) promoter region in human cancers. Large-scale studies of these mutations in multiple tumor types are limited, especially in Asian populations. Analysis of TERT promoter mutations has implications for studying novel tumor types and assessing the function of mutations.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Mutant Analysis

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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