Analysis of THADA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1745-LGZ)

Introduction

Official Full Name: THADA armadillo repeat containing<br />Also known as: GITA; ARMC13; Trm732<br />This gene is the target of 2p21 choromosomal aberrations in benign thyroid adenomas. Single nucleotide polymorphisms (SNPs) in this gene may be associated with type 2 diabetes and polycystic ovary syndrome. The encoded protein is likely involved in the death receptor pathway and apoptosis.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of B4GALNT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2478-LGZ)
Analysis of LINGO4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0443-LGZ)
Analysis of JPH1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1930-LGZ)
Analysis of Canine Parvo Virus (CPV) by Real-Time PCR Method (CAT#: STEM-MB-3034-LGZ)
Analysis of Zika (ZIKV) and Chikungunya (CHIKV) Viruses by RT-qPCR (CAT#: STEM-MT-0065-LGZ)
Analysis of Trypanosoma Evansi by Real-Time PCR Method (CAT#: STEM-MB-2956-LGZ)
Analysis of Uropathogenic Escherichia Coli by Real-Time PCR Method (CAT#: STEM-MB-3074-LGZ)
Analysis of LOC126806579 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1619-LGZ)