Analysis of Thyroglobulin (Human) by ELISA (CAT#: STEM-MB-1219-LGZ)

Introduction

Thyroglobulin (Tg) is a macromolecular glycoprotein secreted by thyroid follicular epithelial cells, most of which are synthesized by thyroid cells and released into the residual cavity of thyroid follicles. Factors such as TSH, iodine deficiency in the thyroid, and thyroid-stimulating immunoglobulin can stimulate its production. In patients with congenital hypothyroidism, Tg detection can differentiate between complete thyroid defect and thyroid hypoplasia. Thyroglobulin is also considered a unique marker of thyroid body integrity. It is a tumor marker of differentiated thyroid cancer (DTC), and can be used as an important reference index for follow-up of patients with differentiated thyroid cancer after treatment. It can also be used to differentiate subacute thyroiditis from pseudo thyrotoxicosis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Metabolic

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant

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