Analysis of TRAF5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0279-LGZ)

Introduction

Official Full Name: TNF receptor associated factor 5<br />Also known as: RNF84; MGC:39780<br />The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family, which contains a meprin and TRAF homology (MATH) domain, a ring-type zinc finger and two TRAF-type zinc fingers. TRAF proteins associate with members of the TNF receptor superfamily and mediate their signal transduction. This protein is part of a multiprotein complex that binds the cytoplasmic domain of the tumor necrosis factor (TNF) receptor and mediates TNF-induced activation. Multiple transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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