Analysis of NSL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0499-LGZ)

Introduction

Official Full Name: NSL1 component of MIS12 kinetochore complex<br />Also known as: DC8; MIS14; C1orf48<br />The gene encodes a protein with two coiled domains that localize to centromeres, chromosome-associated structures that attach to microtubules and mediate chromosome movement during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the kinetochore outer plate. This protein complex is thought to act as a bridge connecting centromere heterochromatin and outer centromere structures. Multiple transcript variants of this gene have been found encoding different isoforms. The 3'UTR region of this gene has a pseudogene on the X chromosome.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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