Analysis of TSPAN32 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2175-LGZ)

Introduction

Official Full Name: tetraspanin 32
Also known as: ART1; PHMX; PHEMX; TSSC6
This gene is a member of the tetraspanin superfamily and is one of several tumor suppressor subtransferable fragments located in the imprinted gene region of chromosome 11p15.5, an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and cancers of the lung, ovary, and breast. This gene is located among several imprinted genes; however, this gene, as well as the tumor suppressor subchromosomal transferable fragment 4, escapes imprinting. The gene may play a role in malignancies and diseases involving this region, which is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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