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Analysis of UBQLN3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2170-LGZ)

Introduction

Official Full Name: ubiquilin 3
Also known as: TUP-1
This gene encodes a ubiquitin-like protein (ubiquilin), which is highly similar to related products of yeast, rat and frog. Ubiquitin contains an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with the proteasome and ubiquitin ligases and are thus thought to functionally link the ubiquitination machinery to the proteasome, thereby affecting protein degradation in vivo. This gene is specifically expressed in the testis. It has been suggested that this gene may regulate cell cycle progression during spermatogenesis, however, the homologous mouse gene has been shown to be essential in embryonic development and spermatogenesis.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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