Analysis of Vancomycin-Resistant Enterococcus (VRE) by Real-Time PCR Method (CAT#: STEM-MB-3825-LGZ)

Introduction

Vancomycin-resistant Enterococci (VRE) are strains of enterococci bacteria that are resistant to the antibiotic vancomycin. Vancomycin is an antibiotic generally prescribed to treat serious infections caused by organisms that are resistant to other antibiotics such as penicillins.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.