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Analysis of VE-Cadherin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0620-LGZ)

Introduction

Cadherin is a transmembrane glycoprotein critical for calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins aggregate to form foci of homophilic binding units. A key determinant of the strength of cadherin-mediated adhesion may be the paramembrane domain of cadherin VE-cadherin (cadherin 5) is the major cadherin found in endothelial cells and plays an important role in angiogenesis and maintenance of barrier permeability.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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