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Analysis of GDF-15 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0203-LGZ)

Introduction

GDF15 is a biomarker for cancer diagnosis, disease progression and prognosis. Its levels are elevated in multiple solid tumor cancer types, and it is a diagnostic biomarker for pancreatic, bowel, ovarian, prostate, primary liver, and lung cancer. It was associated with disease progression, prognosis, and overall survival of a variety of cancers, including colorectal cancer, gastric cancer, hepatocellular carcinoma, non-small cell lung cancer, urothelial/renal cell carcinoma, ovarian cancer, and breast cancer, but not with hematologic tumors.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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