Analysis of VISTA/B7-H5 (Human) by ELISA (CAT#: STEM-MB-1266-LGZ)

Introduction

V-domain Ig suppressor of T cell activation (VISTA) belongs to the B7 family of molecules and shows significant homology with PD-L1 and PD-L2. Unlike other immune checkpoint molecules, VISTA is constitutively expressed on naive T cells and is lost during an immune response; in addition to T cells, VISTA is also expressed on the surface of antigen-presenting cells. VISTA inhibits T-cell activation through T-intracellular and T-extracellular mechanisms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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