Analysis of VPS72 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0757-LGZ)

Introduction

Official Full Name: vacuolar protein sorting 72 homolog
Also known as: YL1; CFL1; Swc2; YL-1; TCFL1
The protein encoded by this gene is a shared subunit of two multicomponent complexes, the histone acetyltransferase complex TRRAP/TIP60, and the chromatin-remodeling SRCAP-containing complex. The TRRAP/TIP60 complex acetylates nucleosomal histones and is critical for transcriptional regulation, double-strand DNA break repair, and apoptosis. The srcap-containing complex catalyzes the exchange of histone H2A with histone variant Htz1 (H2AFZ) into the nucleosome. This protein may be responsible for binding H2AFZ, which plays a role in chromosome segregation. This protein may also play a role in regulating the long-term activity of blood stem cells. Alternative splicing results in multiple transcript variants encoding different protein isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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