Anlysis of Glucose-6-phosphate isomerase (G6PI) by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0805-WXH)

Introduction

Glucose-6-phosphate isomerase (G6PI) catalyses the second step in glycolysis in the reversible interconveision of an aldohexose glucose 6-phosphate, a six membered ring moiety to a ketohexose, fructose 6-phosphate five membered ring moiety. This enzyme is of utmost importance due to its multifunctional role like neuroleukin, autocrine motility factor, etc. in various species. G6PI from Pseudomonas aeruginosa is less explored for its moonlighting properties. These properties can be predicted by studying the active site conservation of residues and their interaction with the specific ligand.