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Ascorbate Peroxidase Analysis in Plant Tissues

Ascorbate peroxidase (APX) belongs to the class I heme-containing peroxidases and can be divided into four categories according to the subcellular localization: cytoplasmic APX, peroxidase APX, chloroplast APX, and mitochondrial APX. APX is an important reactive oxygen species (ROS)-scavenging enzyme which converts H2O2 to H2O and O2 using ascorbate as the specific electron donor. It protects plants from the oxidative damage that can occur as a result of biotic and abiotic stresses. The expression of APX encoding genes as well as amount and activity of APX is differentially modulated by a variety of abiotic stresses in different plant species, such as high temperature, salinity, high light, heavy metals and drought stress.

STEMart provides comprehensive analysis services to identify and investigate the function of APX in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

  • Identification of APX genes
  • Phylogenetic analysis of APX genes
  • Structure analysis of APX genes
  • Expression profile of APX genes of plant under stress conditions
  • 3D structure and physicochemical properties of APX genes
  • Subcellular localization of APX enzymes and Isoenzymes analysis
  • Activity determination of APX

Why Choose Us

  • Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
  • Customized service for plant research
  • Professional instruments and equipment for reliable data and results
  • Rapid turnaround and cost-effective

For more information about our plant APX analysis service, please contact us.

Reference

  1. Caverzan, A., Passaia, G., Rosa, S.B., Ribeiro, C.W., Lazzarotto, F., Margis-Pinheiro, M. (2012). “Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection”. Genet. Mol. Biol. 35, 1011–1019.

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