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Gas isotope ratio mass spectrometry has greatly increasedour understanding of the biogeochemical cycles of C,N, S, and H2O in naturaland agricultural ecosystems. This knowledge is helping to improve crop yieldsand water use,reduce pollution from agricultural chemicals,and documentsources and fluxes of the greenhouse gases. The application of isotope ratio massspectrometry to soil science,in particular,is enhancing our understanding ofecosystem structure and function and facilitating our development of sustainableresource management systems. Automated analysis of stable isotopes of the elements, H,C, N,O, and shas become highly developed, and isotope ratio mass spectrometry can be used for isotope analysis of these elements.