Multiplex up to 5 fluorophores per reaction vessel, preserving precious samples and reagents. Real-time PCR thermal gradients allow for simple and rapid assay optimization. Diverse data analysis and display options provide a comprehensive view of analysis results. Advanced gene expression analysis methods with publication quality statistics and graphs for relative quantitative research. Flexible allele identification module, easy to use in genotyping experiments, automatic sample identification. Embedded tools for endpoint fluorescence analysis allow simple qualitative analysis of sample abundance in single or multicolor analysis. iCycler thermal cycler provides reliable thermal cycling performance The high-performance iQ real-time PCR detection system of the BioRad iCyler iQ PCR Thermal Cycler provides the ideal balance of power and flexibility for a variety of applications. The iQ system matches sensitive filter-based optics to the 96-well block of the BioRad iCycler iQ thermal cycler. The BioRad iCycler iQ Thermal Cycling System is optimized for multiplexed analysis based on high-resolution charge-coupled device (CCD) optical design for highly reproducible quantification of up to 4 targets simultaneously. Data collected from the BioRad iCycler iQ system demonstrates excellent consistency over a wide dynamic range of input sample concentrations
BioRad iCylcer iQ Real-Time PCR-Thermal Cycler Details
The optical module contains excitation system and detection system. The excitation system consists of a fan-cooled 50-watt tungsten-halogen lamp, a thermal filter (infrared-absorbing glass), a 6-position filter wheel housing optical and opacity filter "blanks," and A dual-mirror arrangement that illuminates the entire sample plate. The excitation system of the BioRad iCycler iQ PCR Thermal Cycler is physically located in front of the light module to the right, with the lamps running from right to left perpendicular to the instrument axis. Light is emitted from the lamp, passes through thermal filters and selected color filters, and is reflected by a set of mirrors onto a 96-well plate in a thermal cycler. The light source excites the fluorescent molecules in the well.