BioTek Synergy HT Microplate Reader, BioTek (CAT#: STEM-S-0704-LGZ)

The manufacturer of BioTek Synergy HT Microplate Reader is BioTek. Model is Synergy HT. Biotek Synergy HT is a single channel absorbance, fluorescence and luminescence microplate reader. BioTek's Gen5 or KC4 PC software was used for computer control for all operations, including data restoration and analysis. The Synergy HT is robotically accessible and compatible with BioTek's BioStack Microplate Stacker. When performing fluorescence measurements, the Synergy HT uses a tungsten-quartz halogen lamp combined with a photomultiplier tube (PMT) detector with wavelength-specific interference filters. The low-noise PMT detector measures luminescence through an empty filter in the emission filter wheel. Absorbance measurements were performed by switching to a xenon lamp and a monochromator for wavelength selection. Synergy HT features 4-zone temperature control from ambient 4 °C to 50 °C, readings in 6-, 12-, 24-, 48-, 96- and 384-well microplates are supported, standard 128 x 86 mm geometry size. A shaker plate is internally supported to allow for proper mixing of reagents prior to reading.

Condition: used
Microplate Capacity：
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high
Absorbance mode: plates up to 0.8” (20.30 mm) high
Dimensions (D x W x H): 16 x 15 x 10 in, 40.6 cm x 38 cm x 25.4 cm
Weight: 38 lb. (17 kg)
Environment: Operational temperature 18 ° to 40 °C
Light Source: Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
Humidity: 10% to 85% relative humidity (non-condensing)
Power Consumption: 100 VA max, 130 VA max with injectors
Incubation: Temperature control range from 4° over ambient to 50 °C
Temperature variation ± 0.50 °C across the plate @ 37 °C (250 µL per well with the plate sealed)
Plate Shaking: Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user
Read Modes: Normal, Rapid and Sweep
Absorbance Measurement Range: 0.000 to 4.000 OD
Absorbance Accuracy: 2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates
Absorbance Linearity: 2.000 to 2.500 OD ± 3.0% Normal and Rapid modes, 96- and 384-well plates
Absorbance Repeatability: 0.000 to 2.000 OD ± 1.0% ± 0.005 OD Normal and Rapid modes, 96- and 384-well plates
Fluorescence Read Timing: Excitation Filter: 485/20 nm
Emission Filter: 528/20 nm
96-well read: 89 sec
384-well read: 275 sec
Fluorescence Sensitivity：
5mm probe: 10 pg/mL solution of Sodium Fluorescein in PBS
3mm probe: 20 pg/mL solution of Sodium Fluorescein in PBS
1.5mm probe: 40 pg/mL solution of Sodium Fluorescein in PBS
Time Resolved Fluorescence：
Delay 0 or 20 µs to 16000 µs
Integration interval 20 to 16000 µs
Times adjustable in 10 µs increments

1. Operate using BioTek's Gen5™ or KC4™ data analysis software.
2. Dual optical design with independent fluorescence and absorption channels.
3. 3 mm top and 5 mm bottom fluorescent probes (standard configuration).
4. Optional 1.5 mm top/bottom, 3 mm bottom fluorescent probe (custom configuration).
5. Optional time-resolved fluorescence (TRF) capability (“T” model, such as SIAFRT).
6. Standard low noise PMT.
7. Excitation ¾: 300 to 650 nm (200 to 700 nm with "T" model).
8. Three-quarter emission: 300 to 700 nm.
9. Optional red extended PMT.
10. Excitation ¾: 300 to 650 nm (200 to 800 nm with "T" model).
11. Three-quarter emission: 300 to 800 nm.
12. The absorbance N range is 200 ~ 999 nm.
13. The absorbance OD range is 0.000 ~ 4.000 OD.
14. Low, medium, high and variable rocker speed with adjustable duration.
15. All models read 6-, 12-, 24-, 48-, 96- and 384-well microplates.
16. Injector models are assigned to 6-, 12-, 24-, 48- and 96-well microplates.
17. Operating voltage is 100 to 240 V~(±10%)@ 50 to 60 Hz.
18. 1 serial port (9-pin female).
19. One USB port.
20. Incubate 4-Zone™ to 50 °C.
21. Optional dual-reagent dispensing capability.

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