Characterization of Membrane-Active Peptides by Circular dichroism (CD) (CAT#: STEM-MB-0641-WXH)

Introduction

Peptides that interact with the cell membrane by disrupting it, by passing through it, or by residing at the membrane interface and fusing with it are known as membrane active peptides.

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Principle Applications Procedure Materials Notes Related Products

Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer

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