Dehydroascorbate Reductase Analysis in Plant Tissues

All kinds of abiotic and biotic stresses significantly limit plant productivity by disturbing cellular biochemistry and physiology via the overproduction of activated oxygen molecule-reactive oxygen species (ROS). Uncontrolled accumulation of ROS results in oxidation of DNA and RNA, protein denaturation and decreased enzyme activity as well as lipid peroxidation and carbohydrate oxidation. Within the antioxidant system in plants, the ascorbate-glutathione (AsA-GSH) path-way plays an important role in regulating and controlling the content of ROS.

Dehydroascorbate reductase (DHAR) is located in the chloroplasts, cytosol, mitochondria and peroxisomes. As a regulatory enzyme of the AsA-GSH path-way, DHAR plays a critical role in maintaining ascorbate pool size and redox state by catalyzing the reduction of DHA at the expense of GSH yielding glutathione disulfide (GSSG). The activity of DHAR increases under several stress conditions, such as drought, salinity, ozone, and chilling.

STEMart provides comprehensive analysis services to identify and investigate the function of DHAR in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

• Identification of DHAR genes
• Phylogenetic analysis of DHAR genes
• Structure analysis of DHAR genes
• Expression profile of DHAR genes of plant under stress conditions
• 3D structure and physicochemical properties of DHAR genes
• Subcellular localization of DHAR enzymes and Isoenzymes analysis
• Activity determination of DHAR

Why Choose Us

• Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
• Customized service for plant research
• Professional instruments and equipment for reliable data and results
• Rapid turnaround and cost-effective

For more information about our plant DHAR analysis service, please contact us.