Depiction of the structure of the vitreous body in horses without ocular diseases and in horses with equine recurrent uveitis (ERU) by transmission electron microscopy technology (CAT#: STEM-MIT-0034-LJX)

Introduction

Vitreous body is a glass-like substance in the eyes of human/animal. It is colorless and transparent, semi-solid and gelatinous, and its main component is water. There is no blood vessel in the vitreous body, and the nutrients it needs come from the aqueous humor and choroid, so the metabolism is slow and cannot be regenerated. If there is any defect, the space will be filled by aqueous humor.
Vitreous body plays an important role in the pathogenesis of recurrent uveitis in horses. In vitreous samples from horses with ERU, a large number of dense fibrillary tracts, mononuclear inflammatory cells, and necrotic cells represent the destruction of the vitreous fibrillary network.

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Principle Applications Procedure Materials Notes Related Products

Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
The vitreous body in horses without ocular diseases and in horses with equine recurrent uveitis (ERU)

Notes

Pay attention to air humidity
Voltage needs to be stabilized

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