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Detecting Homozygous Deletions in the CDKN2A(p16INK4a)/ARF(p14ARF) Gene in Urinary Bladder Cancer by RT-qPCR (CAT#: STEM-MT-0021-LGZ)

Introduction

9p21 is an important target in the pathogenesis of bladder cancer. This locus contains the CDKN2A/ARF tumor suppressor gene, which encodes two cell cycle regulatory proteins, cyclin-dependent kinase 2A (p16INK4a) and an alternate reading frame (p14ARF).




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Urinary Bladder Cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements