Detection of Membrane Receptor-Ligand Interactions by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0724-WXH)
Introduction
A myriad of important biological events such as cell signaling are related with cell membrane, and over 50% of drug targets are involved with membrane proteins (e.g., G-protein coupled receptors).
Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.
Applications
NTA has been used by commercial, academic, and government laboratories working with nanoparticle toxicology, drug delivery, exosomes, microvesicles, bacterial membrane vesicles, and other small biological particles, virology and vaccine production, ecotoxicology, protein aggregation, orthopedic implants, inks and pigments, and nanobubbles.
Procedure
1. Sample preparation 2. Nanoparticle Tracking Analysis 3. NTA Data Processing, Display, and Interpretation