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Cobalt (Co), a component of vitamin B12, contributes to the synthesis of hemoglobin and increases the number of red blood cells by stimulating the hematopoietic system of the human bone marrow.<br />Nickel (Ni) is an essential trace element with potential toxicity. Copper (Cu) is one of the indispensable metal elements of the human body. Cu has a function of promoting bone metabolism and has certain antibacterial activities, but excess Cu takes potential risks to the kidney, gastrointestinal tract, movement and sensory nerves. Exposure to high mercury (Hg) likely causes brain damage, inflammation, autoimmunity, and affects the development of the nervous system. Exposure to cadmium (Cd) which was partly produced by industrial emissions or smoking causes kidney and liver toxicity and influences the normal growth of the embryos. This service provides a direct on-line complexion combined with micelle to solvent stacking method for simultaneous determination of metal ions by capillary electrophoresis coupled diode array detector.