Methylation Sensitive Amplification Polymorphism (MSAP) Analysis for DNA Methylation by Capillary Electrophoresis (CAT#: STEM-ET-0309-ZJF)

Introduction

DNA methylation is an epigenetic mechanism that influences gene expression, transposon mobility and genome integrity. Additionally, methylation affects the chromatin structure and controls its condensation in the nucleus. Methylation Sensitive Amplification Polymorphism (MSAP) is one of the most commonly used methods for assessing DNA methylation changes in plants and animals.
Compared with other techniques to determine DNA methylation degree, this service has the following advantages:
1. It does not need to know the sequence information of the tested DNA, and it is universal in different organisms, and can be used in organisms with unknown DNA sequence background knowledge.
2. The operation is relatively simple, and it can be operated without improvement on the basis of AFLP technology system.
3. Cytosine methylation changes at CCGG sites can be detected on a genome-wide scale.

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Principle Applications Procedure Materials Notes Related Products

Principle

Capillary Electrophoresis (CE) is physical method of analysis which performs in a separation channel of elastic quartz capillary, under the influence of a high voltage direct current field. Charged analytes dissolved in an electrolyte solution are separated based on differences in mobility and/or distribution behavior of components. The migration velocity of an analyte under an electric field is determined by the electrophoretic mobility of the analyte and the electro-osmotic mobility of the buffer inside the capillary. The electrophoretic mobility of a solute depends on the characteristics of the solute (electric charge, molecular size and shape) and those of the buffer in which the migration takes place (type and ionic strength of the electrolyte, pH, viscosity and additives). Capillary electrophoresis provides greater resolution, higher sensitivity and online detection. It enables single-cell analysis and even single-molecule analysis, optimizing separation and analysis of biological macromolecules.

Applications

Genomics, plant and animal genome methylation

Procedure

1. Genomic DNA extraction
2. Digestion and ligation
3. PCR amplification
4. Capillary electrophoresis detection
5. Raw data analysis

Materials

• Capillary electrophoresis apparatus
• Sample solution
• Buffer solution

Notes

Sample requirements:
Genomic DNA: Concentration > 20-30ng/ul, OD260/280 about 1.7-2.1, transported by ice pack.
Other sample types and requirements: Contact us

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