Determination of diffusion coefficients in live cells by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0041-WXH)

Introduction

Movement of molecules in and out of cellular compartments or structures is a basic physical phenomenon that is important for many biological processes including transcription, molecular transport, signal transduction, and accumulation of molecules. The diffusion coefficient (also termed diffusivity) is a quantitative measure of a molecule's movement in and out of a cell as well as in solution. The diffusion coefficient is a constant expressing the relationship between flux due to molecular diffusion and the concentration gradient of the molecule of interest.