Determination of target furanocoumarins and flavonoids in cosmetic and pharmaceutical samples by ultra-high performance supercritical fluid chromatography (CAT#: STEM-CT-0057-LJX)

Introduction

Furanocoumarins exist in some Chinese medicine, such as Qianhu, Duhua, Angelica, Angelica angelica, psoralen and so on. Furanocoumarins and their compounds have a strong inhibitory effect on the activity of CYP3A4, which interferes with the metabolism of anticancer drugs and thus affects the efficacy of drugs in vivo.
Flavonoids are now recognized as indispensable ingredients in a variety of nutraceutical, pharmaceutical, pharmaceutical and cosmetic applications. This is attributed to their antioxidant, anti-inflammatory, anti-mutagenic and anti-carcinogenic properties as well as their ability to regulate the function of key cellular enzymes.
Coumarins and flavonoids can be quickly separated by supercritical fluid chromatography.

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Principle Applications Procedure Materials Notes Related Products

Principle

Supercritical fluid is a substance that has both gaseous and liquid properties above the critical point. Supercritical fluids have the advantages of high diffusion coefficient, low viscosity, adjustable solubility and high vapor phase density, so they can provide efficient mass spectrometry ionization and separation results.
Supercritical fluid chromatography (SFC) is an efficient separation technique that uses supercritical fluid as a mobile phase. The samples are packed into short tubes or SPE columns, and the samples are compressed and regulated by supercritical fluid to obtain good solubility. The sample components are then separated by column interaction, thus achieving the separation of different compounds.

Applications

For efficient separation of substances
Widely used in biology, chemistry, environmental protection and other fields

Procedure

1. Sample injection
2. The high pressure pump increases the pressure of the sample and mobile phase
3. The sample and mobile phase enter the chromatographic column
4. Flow limiter assists sample separation

Materials

• Sample Type:
Furanocoumarins and flavonoids

Notes

1. In the process of separation, the control of pressure and temperature of supercritical fluid is very important, which affects the properties and separation efficiency of supercritical fluid.
2. In addition, the selection of the appropriate column, packing and moving equivalent factors will also affect the separation effect.

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