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Determining the freezing point of fruits by Hortvet method (CAT#: STEM-PPA-0160-LJX)

Introduction

Freezing point is one of the important physical characteristics of fruits and vegetables. The freezing point of fruit and vegetable tissue is affected by the type, variety, development degree and cultivation conditions of fruit and vegetable. Measuring the freezing point of fruits and vegetables is helpful to determine the appropriate storage and freezing temperature of fruits and vegetables. However, the freezing point determination of fruit and vegetable tissue is complicated. Since the freezing point of fruit and vegetable juice is slightly higher than the freezing point of fruit and vegetable living tissue, by measuring the freezing point of fruit and vegetable juice, the freezing point of fruit and vegetable living tissue can be reflected to a certain extent.




Principle

The current popular method for detecting the freezing point of milk is the Hortvet cryoscope. A beaded thermistor and a churn device are arranged in the sample tube of the detector. During the test, a small amount of milk sample is put into the sample tube and immediately placed in the cold trap. The temperature of the sample is measured by the thermistor. The sample is rapidly iced and slowly cooled until -3 ℃. A vibrating stirring rod sends energy pulses toward the wall of the sample test tube, forming ice crystals that create a "frozen pulse." As the supercooled milk sample freezes, the potential heat of melting is released, and the temperature of the sample rises to the freezing point plateau, after which the temperature continues to drop. The temperature of the platform is the freezing point of milk.

Applications

For determining the freezing point of liquid

Procedure

1. Put the sample into the test tube
2. Put the test tube into the cold trap
3. Stir and ice the sample quickly with slow cooling until -3 ℃
4. The sample forms ice crystals
5. Read the freezing temperature

Materials

• Sample Type:
Fruits

Notes

The cold trap temperature can be automatically controlled at a constant temperature between -6.5 ℃ and -9.5 ℃.
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