Display myelin sheath by Luxol fast blue method (CAT#: STEM-CT-0010-LJX)

During HE staining of conventional paraffin sections, lipids in the myelin sheath were dissolved by ethanol and xylene, which affected the display of morphological characteristics and composition of the myelin sheath. Luxol fast blue (LFB) is a copper phthalocyanine dye, which can bind to myelin in ethanol solutions. The myelin structure of the nerve tissue can be well demonstrated by Luxol fast blue.

Staining of nerve tissue section samples from experimental animals

1.After the sections were cleaned with pure water, they were put into 0.1% LFB solution and incubated at 56-60℃ for 8-16h.
2.Wash with ethanol
3.Wash with pure water
4.Color separation
5.Redyeing
6.Rinse and dehydrate with n-butanol
7.Xylene transparent
8.Seal the slices

• Sample Type:
Nerve tissue section samples from experimental animals

1.For paraffin sections, dewaxing should be done in xylene first, and then dehydrated in anhydrous ethanol.
2.After staining with LFB, restaining with tar purple solution or HE dye solution can better show the morphology of cells.