Stain rat myelin sheath by Luxol fast blue method (CAT#: STEM-CT-0011-LJX)

Luxol Fast Blue (LFB) belongs to cupric phthalocyanine dye, which usually stains the brain, spinal cord and peripheral nerves. The myelin sheath of nerve is bright blue, the background is colorless to light blue, which can be used to show the morphological structure and pathological changes of the myelin sheath of nerve. The myelin structure of nerve tissue can be well displayed by LFB myelin staining, which is meaningful for pathological diagnosis and study of nerve tissue.

Staining of nerve tissue section samples from experimental animals

1. Dewaxing paraffin sections
2. Clean separately with pure water and ethanol
3. Add Luxol Fast Blue dyeing solution and stain at room temperature;
4. Add ethanol to wash away excess dyeing liquid
5. Rinse with pure water
6. Color separation with Luxol differentiation solution
7. Add ethanol to differentiate gray matter clearly
8. Rinse with pure water
9. Conventional dehydration
10. Xylene transparent
11. Seal the slices

• Sample Type:
Rat myelin sheath

1.For paraffin sections, dewaxing should be done in xylene first, and then dehydrated in anhydrous ethanol.
2.After staining with LFB, restaining with tar purple solution or HE dye solution can better show the morphology of cells.