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Examination of ink by Raman Spectroscopy (CAT#: STEM-ST-0026-WXH)

Introduction

Examination of ink is very important aspect for the investigation of questioned documents. Sometimes it is necessary to apply chemical methods that normally cause partial destruction of the examined material, but Raman spectroscopy provides direct identification of inks on documents for determination of sequence of the handwritten lines. The investigations are mostly often aimed at authenticating the document or at determining its age or origin.
This could be possible due to some characteristics of the ink such as luminescence in visible or in infrared light. A good discrimination between inks has been obtained using Raman spectroscopy, possibly used to differentiate between inks of the same colour.




Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope
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