Study of halophile by Raman Spectroscopy (CAT#: STEM-ST-0096-WXH)
Introduction
Halophiles are microorganisms that require certain concentrations of salt to survive, and they are found in both Eubacterial and Archaeal domains of life.
Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions. The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.
Applications
• Analysis of biocompatibility of a material. • Analysis of nucleic acids. • Study of interactions between drugs and cells. • Photodynamic therapy (PDT). • Analyzing metabolic accumulations of a substance or compounds. • Diagnosis of disease. • Analysis of individual cells. • Cell sorting applications. • Analyzing the features of biomolecules. • Study of bone structure.
Procedure
1. Preparation of samples 2. Determine instrument parameters 3. Perform background scan 4. Test the sample 5. Data analysis